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Sample GSM62979 Query DataSets for GSM62979
Status Public on Jul 12, 2006
Title CD8+Tet-_03
Sample type RNA
Source name Spleen Tcells day 14
Organism Mus musculus
Characteristics Strain: B10.C-H7b(47N)/SN
Gender: Female
Age: 6-8wks
Biomaterial provider Dr. Claude Perreault
Treatment protocol Immunization of a female B10.C-H7b(47N)/SN with splenocytes from male B10.C-H7b(47N)/SN + male C57BL/10Snj
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from sorted populations using TRIzol (Invitrogen/Life Technologies)
Label biotin + R-phycoerythrin streptavidin
Label protocol Labeling of samples was performed according to standard Affymetrix protocol
Hybridization protocol EukGE-WS2v5_450
Scan protocol Pixel Size 1.56
Filter 570
Scan Temperature
Scan Date Aug 30 2004 05:59PM
Scanner ID 50207280
Number of Scans 1
Scanner Type M10
Description B10.C-H7b(47N)/SN female mice were immunized by i.p. co-injection of C57BL/10Snj male and B10.C-H7b(47N)/SN male spleen cells. Fourteen days later, single-cell suspensions were prepared from the spleen of immunized mice. CD8+H7aTet+, CD8+HYTet+ and CD8+Tet- cells were sorted using a FACSVantage SE with Diva option (BD Biosciences, San Jose). Purity of sorted cell populations was always ≥ 98%. Tet+ populations containing between 8-300 thousand cells and Tet- population containing 2-3 million cells were obtained for each mouse and processed separately.
Data processing MAS5
Submission date Jul 05, 2005
Last update date Sep 08, 2006
Contact name Etienne Caron
Organization name Hopital Maisonneuve Rosemont
Department Recherche
Lab Dr Claude Perreault
Street address 5415 Boul. L'Assomption
City Montreal
State/province Quebec
ZIP/Postal code H1T 2M4
Country Canada
Platform ID GPL1261
Series (1)
GSE2924 Immunodomination leads to selective expansion of the fittest CD8 T cells

Data table header descriptions
VALUE signal a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)

Data table
AFFX-BioB-5_at 2006.1 P
AFFX-BioB-M_at 3248.6 P
AFFX-BioB-3_at 2781.7 P
AFFX-BioC-5_at 10227 P
AFFX-BioC-3_at 9202.4 P
AFFX-BioDn-5_at 27525.7 P
AFFX-BioDn-3_at 40391.4 P
AFFX-CreX-5_at 102849.5 P
AFFX-CreX-3_at 107605.1 P
AFFX-DapX-5_at 22.2 A
AFFX-DapX-M_at 140.9 A
AFFX-DapX-3_at 69.7 A
AFFX-LysX-5_at 17.2 A
AFFX-LysX-M_at 77.2 A
AFFX-LysX-3_at 5 A
AFFX-PheX-5_at 5.6 A
AFFX-PheX-M_at 94.1 A
AFFX-PheX-3_at 20.9 A
AFFX-ThrX-5_at 37.4 A
AFFX-ThrX-M_at 8.1 A

Total number of rows: 45101

Table truncated, full table size 819 Kbytes.

Supplementary data files not provided

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