|
| Status |
Public on Feb 25, 2012 |
| Title |
Myc_WRN_E6 (Bioconductor lumi R) |
| Sample type |
RNA |
| |
|
| Source name |
murine lymphoma
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B6
disease state: B cell lymphoma
genotype/variaton: Eµ-Myc Wrn Dhel/Dhel
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 4
|
| Data processing |
The data were normalised using quantile normalisation and Affy-like background subtraction (Genes with signal/noise ratio <3 eliminated from further analysis) with Bioconductor lumi in R (Normalized I)
|
| |
|
| Submission date |
Nov 29, 2010 |
| Last update date |
Feb 25, 2012 |
| Contact name |
Russell Moser |
| E-mail(s) |
rmoser@fhcrc.org
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Human Biology
|
| Lab |
Grandori Laboratory
|
| Street address |
1100 Fairview Avenue N.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE25671 |
Genome-wide analysis of gene expression in Eµ-Myc and Eµ-Myc Wrn Δhel/Δhel murine lymphomas |
|
