|
| Status |
Public on Jan 01, 2011 |
| Title |
DE5_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
striatum
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: DE5-Tg
tissue: striatum
age: 14 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
DE033
|
| Data processing |
Slides were scanned using Illumina BeadStation and signal extracted using Illumina BeadStudio software. Raw data is the output of the BeadStudio software and was analyzed using Bioconductor packages. Data was normalized using the LIMMA package.
|
| |
|
| Submission date |
Nov 29, 2010 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Elizabeth A. Thomas |
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular Biology
|
| Street address |
10550 N. Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE25232 |
In vivo cell-autonomous transcriptional abnormalities revealed in mice expressing mutant huntingtin in striatal but not cortical neurons |
|
