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Sample GSM631423 Query DataSets for GSM631423
Status Public on Jan 03, 2011
Title BMDM stimulated with cmB16F1, rep1
Sample type RNA
Source name BMDM stimulated with cmB16F1
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: bone marrow cells after a total of 7 days cultivation in vitro
stimulation: cmB16
Treatment protocol At day 4 the culture medium was replaced by stimulation medium as indicated and cells were cultured for 3 more days.
Growth protocol Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin].
Extracted molecule total RNA
Extraction protocol Total RNA extraction was carried out with a RNeasy RNA-extraction Kit (Qiagen) according to the manufacturers protocols
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description mouse bone marrow cells after a total of 7 days cultivation in vitro stimulated with cmB16F1, rep1
Data processing The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. A custom CDF (Mouse4302_Mm_UG, Brainarray Version 9) was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
Submission date Nov 30, 2010
Last update date Jan 03, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
Platform ID GPL6526
Series (1)
GSE25707 Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

Data table header descriptions
VALUE RMA signal intensity

Data table
AFFX-18SRNAMur/X00686_3_at 9.24282617
AFFX-18SRNAMur/X00686_5_at 8.18034927
AFFX-18SRNAMur/X00686_M_at 8.77696065
AFFX-b-ActinMur/M12481_3_at 14.0062126
AFFX-b-ActinMur/M12481_5_at 13.2794507
AFFX-b-ActinMur/M12481_M_at 13.3751422
AFFX-BioB-3_at 7.9521682
AFFX-BioB-5_at 7.82895948
AFFX-BioB-M_at 8.13611411
AFFX-BioC-3_at 9.13899465
AFFX-BioC-5_at 8.88962036
AFFX-BioDn-3_at 10.9996873
AFFX-BioDn-5_at 9.76247483
AFFX-CreX-3_at 12.4009426
AFFX-CreX-5_at 12.0374003
AFFX-DapX-3_at 6.48544357
AFFX-DapX-5_at 6.26244991
AFFX-DapX-M_at 6.42488793
AFFX-GapdhMur/M32599_3_at 13.1317407
AFFX-GapdhMur/M32599_5_at 12.9389719

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.

Supplementary file Size Download File type/resource
GSM631423.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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