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Sample GSM631424 Query DataSets for GSM631424
Status Public on Jan 03, 2011
Title BMDM stimulated with dexa+IL4, rep2
Sample type RNA
 
Source name BMDM stimulated with dexa+IL4
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: bone marrow cells after a total of 7 days cultivation in vitro
stimulation: Dexa+IL4
Treatment protocol At day 4 the culture medium was replaced by stimulation medium as indicated and cells were cultured for 3 more days.
Growth protocol Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin].
Extracted molecule total RNA
Extraction protocol Total RNA extraction was carried out with a RNeasy RNA-extraction Kit (Qiagen) according to the manufacturers protocols
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description mouse bone marrow cells after a total of 7 days cultivation in vitro stimulated with dexa+IL4, rep2
Data processing The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. A custom CDF (Mouse4302_Mm_UG, Brainarray Version 9) was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Nov 30, 2010
Last update date Jan 03, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL6526
Series (1)
GSE25707 Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 9.16818425
AFFX-18SRNAMur/X00686_5_at 8.14392356
AFFX-18SRNAMur/X00686_M_at 8.68888525
AFFX-b-ActinMur/M12481_3_at 13.7519457
AFFX-b-ActinMur/M12481_5_at 12.8242625
AFFX-b-ActinMur/M12481_M_at 12.9472125
AFFX-BioB-3_at 8.25996672
AFFX-BioB-5_at 7.96247106
AFFX-BioB-M_at 8.3946926
AFFX-BioC-3_at 9.41837624
AFFX-BioC-5_at 9.17638991
AFFX-BioDn-3_at 11.1462145
AFFX-BioDn-5_at 10.0599326
AFFX-CreX-3_at 12.5419881
AFFX-CreX-5_at 12.2285361
AFFX-DapX-3_at 6.49497817
AFFX-DapX-5_at 6.30561096
AFFX-DapX-M_at 6.4780804
AFFX-GapdhMur/M32599_3_at 12.2490023
AFFX-GapdhMur/M32599_5_at 11.9913844

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM631424.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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