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Sample GSM631425 Query DataSets for GSM631425
Status Public on Jan 03, 2011
Title BMDM stimulated with cmB16F1 and dexa+IL4, rep2
Sample type RNA
 
Source name BMDM stimulated with cmB16F1 and dexa+IL4
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: bone marrow cells after a total of 7 days cultivation in vitro
stimulation: cmB16+Dexa+IL4
Treatment protocol At day 4 the culture medium was replaced by stimulation medium as indicated and cells were cultured for 3 more days.
Growth protocol Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin].
Extracted molecule total RNA
Extraction protocol Total RNA extraction was carried out with a RNeasy RNA-extraction Kit (Qiagen) according to the manufacturers protocols
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description mouse bone marrow cells after a total of 7 days cultivation in vitro stimulated with cmB16F1 and dexa+IL4, rep2
Data processing The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. A custom CDF (Mouse4302_Mm_UG, Brainarray Version 9) was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Nov 30, 2010
Last update date Jan 03, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL6526
Series (1)
GSE25707 Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 9.97927378
AFFX-18SRNAMur/X00686_5_at 8.43250241
AFFX-18SRNAMur/X00686_M_at 9.30176842
AFFX-b-ActinMur/M12481_3_at 13.6975257
AFFX-b-ActinMur/M12481_5_at 12.30766
AFFX-b-ActinMur/M12481_M_at 12.6371409
AFFX-BioB-3_at 8.43212576
AFFX-BioB-5_at 8.18802324
AFFX-BioB-M_at 8.656824
AFFX-BioC-3_at 9.73061888
AFFX-BioC-5_at 9.49701943
AFFX-BioDn-3_at 11.6270469
AFFX-BioDn-5_at 10.4257101
AFFX-CreX-3_at 12.8364351
AFFX-CreX-5_at 12.6280374
AFFX-DapX-3_at 6.41917748
AFFX-DapX-5_at 6.32334923
AFFX-DapX-M_at 6.47072253
AFFX-GapdhMur/M32599_3_at 12.9612275
AFFX-GapdhMur/M32599_5_at 12.3685761

Total number of rows: 17931

Table truncated, full table size 411 Kbytes.




Supplementary file Size Download File type/resource
GSM631425.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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