NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM631426 Query DataSets for GSM631426
Status Public on Jan 03, 2011
Title BMDM stimulated with cmB16F1, rep2
Sample type RNA
 
Source name BMDM stimulated with cmB16F1
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: bone marrow cells after a total of 7 days cultivation in vitro
stimulation: cmB16
Treatment protocol At day 4 the culture medium was replaced by stimulation medium as indicated and cells were cultured for 3 more days.
Growth protocol Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin].
Extracted molecule total RNA
Extraction protocol Total RNA extraction was carried out with a RNeasy RNA-extraction Kit (Qiagen) according to the manufacturers protocols
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description mouse bone marrow cells after a total of 7 days cultivation in vitro stimulated with cmB16F1, rep2
Data processing The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. A custom CDF (Mouse4302_Mm_UG, Brainarray Version 9) was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Nov 30, 2010
Last update date Jan 03, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL6526
Series (1)
GSE25707 Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 10.5849615
AFFX-18SRNAMur/X00686_5_at 9.16989013
AFFX-18SRNAMur/X00686_M_at 9.77779489
AFFX-b-ActinMur/M12481_3_at 13.9143412
AFFX-b-ActinMur/M12481_5_at 12.7812142
AFFX-b-ActinMur/M12481_M_at 13.051763
AFFX-BioB-3_at 8.05564311
AFFX-BioB-5_at 7.92390589
AFFX-BioB-M_at 8.30988221
AFFX-BioC-3_at 9.38667406
AFFX-BioC-5_at 9.15505606
AFFX-BioDn-3_at 11.2121848
AFFX-BioDn-5_at 9.93776282
AFFX-CreX-3_at 12.55505
AFFX-CreX-5_at 12.2240936
AFFX-DapX-3_at 6.33963907
AFFX-DapX-5_at 6.35244531
AFFX-DapX-M_at 6.43407205
AFFX-GapdhMur/M32599_3_at 13.2030986
AFFX-GapdhMur/M32599_5_at 12.6318935

Total number of rows: 17931

Table truncated, full table size 411 Kbytes.




Supplementary file Size Download File type/resource
GSM631426.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap