|
| Status |
Public on Jan 03, 2011 |
| Title |
BMDM stimulated with dexa+IL4, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM stimulated with dexa+IL4
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: bone marrow cells after a total of 7 days cultivation in vitro
stimulation: Dexa+IL4
|
| Treatment protocol |
At day 4 the culture medium was replaced by stimulation medium as indicated and cells were cultured for 3 more days.
|
| Growth protocol |
Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin].
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was carried out with a RNeasy RNA-extraction Kit (Qiagen) according to the manufacturers protocols
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
mouse bone marrow cells after a total of 7 days cultivation in vitro stimulated with dexa+IL4, rep3
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. A custom CDF (Mouse4302_Mm_UG, Brainarray Version 9) was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Nov 30, 2010 |
| Last update date |
Jan 03, 2011 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6526 |
| Series (1) |
| GSE25707 |
Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A |
|
