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Sample GSM631427 Query DataSets for GSM631427
Status Public on Jan 03, 2011
Title BMDM stimulated with dexa+IL4, rep3
Sample type RNA
 
Source name BMDM stimulated with dexa+IL4
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: bone marrow cells after a total of 7 days cultivation in vitro
stimulation: Dexa+IL4
Treatment protocol At day 4 the culture medium was replaced by stimulation medium as indicated and cells were cultured for 3 more days.
Growth protocol Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin].
Extracted molecule total RNA
Extraction protocol Total RNA extraction was carried out with a RNeasy RNA-extraction Kit (Qiagen) according to the manufacturers protocols
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description mouse bone marrow cells after a total of 7 days cultivation in vitro stimulated with dexa+IL4, rep3
Data processing The data were analyzed with a commercial software called JMP Genomics, version 3.2, from SAS. A custom CDF (Mouse4302_Mm_UG, Brainarray Version 9) was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Nov 30, 2010
Last update date Jan 03, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL6526
Series (1)
GSE25707 Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-18SRNAMur/X00686_3_at 9.28632151
AFFX-18SRNAMur/X00686_5_at 7.98531921
AFFX-18SRNAMur/X00686_M_at 8.67211888
AFFX-b-ActinMur/M12481_3_at 13.5768564
AFFX-b-ActinMur/M12481_5_at 12.4546954
AFFX-b-ActinMur/M12481_M_at 12.6323473
AFFX-BioB-3_at 8.26629124
AFFX-BioB-5_at 8.00090024
AFFX-BioB-M_at 8.39562104
AFFX-BioC-3_at 9.37002399
AFFX-BioC-5_at 9.13164102
AFFX-BioDn-3_at 11.0810224
AFFX-BioDn-5_at 9.90324752
AFFX-CreX-3_at 12.3716584
AFFX-CreX-5_at 12.0435918
AFFX-DapX-3_at 6.54792883
AFFX-DapX-5_at 6.39434402
AFFX-DapX-M_at 6.50495928
AFFX-GapdhMur/M32599_3_at 12.1869911
AFFX-GapdhMur/M32599_5_at 11.7903506

Total number of rows: 17931

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM631427.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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