|
| Status |
Public on Jan 01, 2012 |
| Title |
LPS & HBD3 rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow derived macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
cell type: Bone marrow derived macrophages
treatment: LPS & hBD3
time: 1 hour
|
| Treatment protocol |
Macrophages at a concentration 5 x 10E5 cells per well (6 well plate) were incubated overnight in the absence of M-CSF. Cells were treated as described above, then incubated for 1 hour at 37 degrees.
|
| Growth protocol |
Bone marrow was flushed from femurs of Balb/c mice and incubated for 7-10 days in the presence of macrophage colony stimulating factor (M-CSF, 20ng/ml)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNA bee (AMS Biotechnology)
|
| Label |
biotin
|
| Label protocol |
Amplified, biotinylated cRNA was synthesized using Illumina® TotalPrep Amplification Kit (Ambion, Austin, Texas)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
LPS & HBD3 rep 1
|
| Data processing |
The data were normalised using quantile normalisation with LIMMA in R
|
| |
|
| Submission date |
Dec 06, 2010 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Graeme Richard Grimes |
| E-mail(s) |
graeme.grimes@ed.ac.uk
|
| Organization name |
University of Edinburgh, The Institute of Genetics and Cancer
|
| Department |
Human Genetics Unit
|
| Lab |
Bioinformatics Analysis Core
|
| Street address |
Western General Hospital, Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE25870 |
Human Beta-Defensin 3 attenuates the pro-inflammatory effects of LPS at 1 hour |
| GSE25873 |
Effect of hBD3 on transcription in TLR4-stimulated macrophages |
|
