|
Status |
Public on Dec 10, 2017 |
Title |
Regenerating_MM13 |
Sample type |
RNA |
|
|
Source name |
Laser captured spinal cord neurons
|
Organism |
Rattus norvegicus |
Characteristics |
Fischer 344, adult female
|
Biomaterial provider |
Lawrence Moon
|
Treatment protocol |
Cut 10um transverse cryosections of unfixed spinal cord, dehydrate, laser capture
|
Growth protocol |
n/a
|
Extracted molecule |
total RNA |
Extraction protocol |
PicoPure (version C), Arcturus
|
Label |
Biotin
|
Label protocol |
Three round Eberwine amplification, proprietary modified Message Amp using BioArray High Yield labeling kit (Enzo) in last round.
|
|
|
Hybridization protocol |
Standard Affymetrix procedure for fragmentation and hybridization
|
Scan protocol |
Standard GCOS scanning, GeneChip Scanner 3000
|
Description |
Identification of genes associated with axotomy or regeneration in the CNS.
|
Data processing |
GCOS, scaled to 200
|
|
|
Submission date |
Jul 12, 2005 |
Last update date |
Dec 10, 2017 |
Contact name |
Lawrence DF Moon |
E-mail(s) |
lawrence.moon@kcl.ac.uk
|
Organization name |
King's College London
|
Department |
Wolfson Centre for Age-Related Diseases
|
Lab |
Moon team
|
Street address |
16 - 18 Newcomen Street
|
City |
London |
ZIP/Postal code |
SE11UL |
Country |
United Kingdom |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE2925 |
Identification of genes expressed by spinal cord neurons after axotomy and during axon regeneration |
|