|
| Status |
Public on Jun 07, 2011 |
| Title |
Tendon_lesional_rep20 |
| Sample type |
RNA |
| |
|
| Source name |
Tendon;Suspraspinatus_Lesional
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: Tendon;Suspraspinatus
age: 46
donor: 32
disease state: Lesional
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by RNEasy purification was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
042209_E6_Restart
|
| Data processing |
The data were analyzed with Microarray Suite GCOS using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 14, 2010 |
| Last update date |
Jun 07, 2011 |
| Contact name |
Scott Jelinsky |
| E-mail(s) |
Scott.Jelinsky@pfizer.com
|
| Phone |
617-674-7272
|
| Organization name |
Pfizer
|
| Department |
Inflammation and Immunology
|
| Lab |
Computational Precision Medicine
|
| Street address |
610 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26051 |
Analysis of Human Tendinopathy Gene Expression |
|
