|
Status |
Public on Feb 01, 2011 |
Title |
RbBP5 shRNA, undifferentiated |
Sample type |
RNA |
|
|
Source name |
E14 cells
|
Organism |
Mus musculus |
Characteristics |
shRNA: RbBP5 knockdown differentiation status: undifferentiated
|
Treatment protocol |
Cells stably infected with viruses expressing indicated shRNAs were either untreated or treated with retinoic acid (RA) for differentiation (four days depletion of LIF followed by four days of 1 µM all-trans RA on monolayer culture.
|
Growth protocol |
E14 cells were cultured on 0.1% gelatinized tissue-culture plates in complete ESC growth medium supplemented with LIF
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using the RNeasy kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
RbBP5 shRNA, undifferentiated 4639127062_C.txt
|
Data processing |
Results were analyzed by GeneSpring GX 10.0.2. Genes were filtered on Flags Present or Marginal. Signals were normalized against the median in each sample.
|
|
|
Submission date |
Dec 17, 2010 |
Last update date |
Feb 01, 2011 |
Contact name |
Hao Jiang |
E-mail(s) |
hjiang@rockefeller.edu
|
Organization name |
Rockefeller University
|
Lab |
Roeder Lab
|
Street address |
1230 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE26136 |
Mammalian Dpy-30 regulates genomic H3K4 methylation and is essential for ES cell fate specification |
|