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Sample GSM647571 Query DataSets for GSM647571
Status Public on Jan 13, 2011
Title Septic Shock_biological rep38
Sample type RNA
 
Source name Children ≤ 10 years old
Organism Homo sapiens
Characteristics tissue: Whole Blood
disease state: septic shock patient
age (years): 7
outcome: Survivor
Treatment protocol standard ICU care for septic shock
Extracted molecule total RNA
Extraction protocol Qiagen extraction from PaxGene whole blood RNA collection tubes
Label biotin
Label protocol WT-Ovation Pico (NuGen)
 
Hybridization protocol Standard Affymetrix
Scan protocol Affymetrix GeneChip Scanner 3000
Description Gene expression data from septic shock patient
Data processing Gene expression was measured using one patient sample per chip. Image files were captured using an Affymetrix GeneChip Scanner 3000. CEL files were subsequently preprocessed using Robust Multiple-Array Average (RMA) normalization and GeneSpring GX 7.3 software (Agilent Technologies, Palo Alto, CA). All signal intensity-based data were used after RMA normalization, which specifically suppresses all but significant variation among lower intensity probe sets. All chips representing patient samples were then normalized to the respective median values of controls.
 
Submission date Dec 30, 2010
Last update date Feb 23, 2015
Contact name Hector R Wong
E-mail(s) hector.wong@cchmc.org
Phone 513-636-4259
Fax 513-636-4267
Organization name Cincinnati Children's Hospital Medical Center
Department Pediatrics
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL570
Series (1)
GSE26378 Expression data from validation cohort of children with septic shock
Relations
Reanalyzed by GSM1617671

Data table header descriptions
ID_REF
VALUE RMA signal GeneSpring GX 7.3 normalized to median of control probes

Data table
ID_REF VALUE
1007_s_at 0.763
1053_at 1.057
117_at 1.27
121_at 1.204
1255_g_at 1.209
1294_at 0.806
1316_at 0.722
1320_at 1.098
1405_i_at 0.802
1431_at 0.987
1438_at 0.979
1487_at 0.688
1494_f_at 1.083
1552256_a_at 0.536
1552257_a_at 0.602
1552258_at 1.117
1552261_at 1.377
1552263_at 0.778
1552264_a_at 0.907
1552266_at 0.988

Total number of rows: 54675

Table truncated, full table size 891 Kbytes.




Supplementary file Size Download File type/resource
GSM647571_13_0004.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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