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Sample GSM647587 Query DataSets for GSM647587
Status Public on Jan 13, 2011
Title Septic Shock_biological rep53
Sample type RNA
 
Source name Children ≤ 10 years old
Organism Homo sapiens
Characteristics tissue: Whole Blood
disease state: septic shock patient
age (years): 9.3
outcome: Survivor
Treatment protocol standard ICU care for septic shock
Extracted molecule total RNA
Extraction protocol Qiagen extraction from PaxGene whole blood RNA collection tubes
Label biotin
Label protocol WT-Ovation Pico (NuGen)
 
Hybridization protocol Standard Affymetrix
Scan protocol Affymetrix GeneChip Scanner 3000
Description Gene expression data from septic shock patient
Data processing Gene expression was measured using one patient sample per chip. Image files were captured using an Affymetrix GeneChip Scanner 3000. CEL files were subsequently preprocessed using Robust Multiple-Array Average (RMA) normalization and GeneSpring GX 7.3 software (Agilent Technologies, Palo Alto, CA). All signal intensity-based data were used after RMA normalization, which specifically suppresses all but significant variation among lower intensity probe sets. All chips representing patient samples were then normalized to the respective median values of controls.
 
Submission date Dec 30, 2010
Last update date Feb 23, 2015
Contact name Hector R Wong
E-mail(s) hector.wong@cchmc.org
Phone 513-636-4259
Fax 513-636-4267
Organization name Cincinnati Children's Hospital Medical Center
Department Pediatrics
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL570
Series (1)
GSE26378 Expression data from validation cohort of children with septic shock
Relations
Reanalyzed by GSM1617686

Data table header descriptions
ID_REF
VALUE RMA signal GeneSpring GX 7.3 normalized to median of control probes

Data table
ID_REF VALUE
1007_s_at 0.614
1053_at 1.367
117_at 4.574
121_at 0.974
1255_g_at 1.217
1294_at 1.634
1316_at 1.029
1320_at 1.022
1405_i_at 0.1
1431_at 1.022
1438_at 0.919
1487_at 1.394
1494_f_at 0.966
1552256_a_at 0.661
1552257_a_at 1.75
1552258_at 2.471
1552261_at 1.067
1552263_at 2.022
1552264_a_at 1.512
1552266_at 1.113

Total number of rows: 54675

Table truncated, full table size 891 Kbytes.




Supplementary file Size Download File type/resource
GSM647587_20_0007.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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