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Sample GSM64935 Query DataSets for GSM64935
Status Public on Jul 22, 2005
Title R1 EB 12h 1, chip MOE430B
Sample type RNA
 
Source name R1, Fetal cells
Organism Mus musculus
Characteristics Strain: 129/Sv x 129/Sv-CP
Gender: Male
Biomaterial provider Contact details for sample vendor: R1 sample obtained from Andras Nagy's lab
Growth protocol Growth medium: ES Cell Medium - DMEM (high glucose)
Media supplements: Hyclone FCS (heat inactivated), non-essential amino acids, Glutamax, Penicillin/Streptomycin, sodium pyruvate, beta-mercaptoethanol
Culture conditions: 37 C, 5% CO2
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Culturing
Contaminating cell type: none
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID: E165
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=165
SCGP Sample ID: S208
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=165#SAMPLE_167
Short description: 12 hours of an 11-point time course study of R1 embryonic stem cells and their cognate differentiated embryoid bodies. All samples investigated in triplicate. The cells are differentiating embryonic bodies from murine embryonic stem cells.
Estimated purity: 100%
RNA concentration: 2.0 ug/uL
Num cells for RNA prep: Unable to break up embryoid bodies to do a cell count.
Sample volume: 15 uL
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Jul 21, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL340
Series (1)
GSE2972 R1 Embryonic Stem Cell and Embryoid Body Time Course

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 12055.9 P 0.000389797
AFFX-BioB-M_at 23467 P 5.16732e-05
AFFX-BioB-3_at 10877 P 0.000146581
AFFX-BioC-5_at 30968.2 P 7.00668e-05
AFFX-BioC-3_at 23978.8 P 4.42873e-05
AFFX-BioDn-5_at 30555.6 P 4.42873e-05
AFFX-BioDn-3_at 167290 P 7.00668e-05
AFFX-CreX-5_at 324274 P 4.42873e-05
AFFX-CreX-3_at 475290 P 4.42873e-05
AFFX-DapX-5_at 154183 P 4.42873e-05
AFFX-DapX-M_at 318110 P 5.16732e-05
AFFX-DapX-3_at 410459 P 4.42873e-05
AFFX-LysX-5_at 44001.3 P 4.42873e-05
AFFX-LysX-M_at 77068.3 P 4.42873e-05
AFFX-LysX-3_at 125062 P 4.42873e-05
AFFX-PheX-5_at 13967.3 P 4.42873e-05
AFFX-PheX-M_at 17768.8 P 4.42873e-05
AFFX-PheX-3_at 28887.3 P 4.42873e-05
AFFX-ThrX-5_at 5287.38 P 0.000389797
AFFX-ThrX-M_at 7959.15 P 0.000340305

Total number of rows: 22575

Table truncated, full table size 666 Kbytes.




Supplementary file Size Download File type/resource
GSM64935.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM64935.EXP.gz 492 b (ftp)(http) EXP

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