|
| Status |
Public on Jan 20, 2011 |
| Title |
NT_6 |
| Sample type |
RNA |
| |
|
| Source name |
TRAMP Mouse 6
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
cell type: dendritic cells
genotype/variation: TRAMP Transgenic
|
| Treatment protocol |
Cells were purified from digested prostates by magnetic beads >90% purity was confirmed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the qiagen RNAeasy mini spin colomn according to manufacturer's instuctions.
RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
|
| Label |
biotin
|
| Label protocol |
200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol
|
| |
|
| Hybridization protocol |
replicates of each group were hybridized to Affymetrix mouse Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs
|
| Scan protocol |
Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000
|
| Description |
Dendritic cells Tumor Bearing TRAMP mouse 6
|
| Data processing |
Data were collected using Affymetrix AGCC software. Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm.
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jan 20, 2011 |
| Contact name |
Stephanie Kaye Watkins |
| E-mail(s) |
watkinssk@mail.nih.gov
|
| Phone |
301-846-6871
|
| Fax |
301-846-6005
|
| Organization name |
NCI-Frederick
|
| Department |
CIP/LMI
|
| Lab |
Tumor Immunity and Tolerance
|
| Street address |
1050 Boyles St.
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE26719 |
Gene expression analysis of dendritic cells from normal or tumor bearing prostates |
|
