|
| Status |
Public on Sep 04, 2005 |
| Title |
PHYAPHYBDARK2 ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Dark grown seedlings, Ecotype: columbia. Alleles phyA-211 and phyB-9
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
phyA phyB double mutants seeds were sown on 0.8% (w/v) agar in clear plastic boxes and incubated at 6 degrees for 5 d. Chilled seeds were exposed to 1 h of white light at 22 degrees to induce homogeneous seed germination. After 3 d in full D at 22 degrees seedlings were harvested in liquid nitrogen and total RNA was extracted by using the RNEASY Plant mini kit (Qiagen).
|
| Treatment protocol |
phyAphyB double mutant seeds incubated at 6 degrees for 5 d. Chilled seeds were exposed to 1 h of white light at 22 degrees to induce homogeneous seed germination. After 3 d in full D at 22 degrees seedlings were harvested
|
| Growth protocol |
seeds were sown on 0.8% (w/v) agar
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy plant mini kit (Qiagen)
|
| Label |
Affymetrix instructions
|
| Label protocol |
Affymetrix instructions
|
| |
|
| Hybridization protocol |
Affymetrix instruction. Hybridization to ATH1 Affymetrix Arabidopsis Gene Chips
|
| Scan protocol |
Affymetrix instructions
|
| Description |
For more information see Mazzella et al, 2005. Plant Cell Vol 17 (september)
|
| Data processing |
the scaling tab of the Affymetrix microarray suite in the mode “all probe sets” was used to standardize the trimmed mean signal of each array to the “target signal” according to manufacturer instructions.
|
| |
|
| Submission date |
Jul 27, 2005 |
| Last update date |
Oct 28, 2005 |
| Contact name |
Maria Agustina Mazzella |
| E-mail(s) |
mazzella@dna.uba.ar
|
| Phone |
54-11-47832871
|
| Organization name |
INGEBI
|
| Street address |
Vuelta de Obligado 2490
|
| City |
Capital Federal |
| State/province |
Buenos AIres |
| ZIP/Postal code |
1428 |
| Country |
Argentina |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE3038 |
ATH1 Transcriptome profiling of dark grown photoreceptor mutants seedlings of Arabidopsis |
|
