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Sample GSM661375 Query DataSets for GSM661375
Status Public on Jan 27, 2011
Title Sham treated group 4
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
treatment time: 2h
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description 5120221010_C
Sham treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Jan 25, 2011
Last update date Jan 27, 2011
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE26864 Gene expression in mouse lung epithelial cells treated with synthetic TLR ligands to induce resistance

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.
Avg_NBEADS
BEAD_STDERR
DETECTION PVAL

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR DETECTION PVAL
ILMN_2896528 143.6265 48 11.29134 0
ILMN_2721178 31.51567 45 7.373973 0.02255639
ILMN_3033922 1200.26 43 48.37694 0
ILMN_3092673 5985.732 45 254.6255 0
ILMN_2816356 76.53875 33 10.42514 0
ILMN_2808939 988.8544 45 64.07905 0
ILMN_2634564 654.9033 38 38.70223 0
ILMN_2737647 2.966496 33 6.142998 0.5626566
ILMN_2734484 646.2751 44 29.2571 0
ILMN_2952292 233.0169 47 14.17887 0
ILMN_2699078 -8.299436 36 4.188006 0.9423559
ILMN_1213681 437.4788 46 25.65462 0
ILMN_2735413 0.9201222 30 5.340725 0.6516291
ILMN_2735415 12.44638 31 7.31047 0.2406015
ILMN_2891688 814.3455 33 44.3126 0
ILMN_2637698 1708.99 44 77.30938 0
ILMN_2674228 527.6934 40 42.97683 0
ILMN_2601546 37.7486 40 9.897831 0.0112782
ILMN_1230831 44.2435 33 10.10454 0.00877193
ILMN_2848071 30.43539 29 9.518237 0.02380952

Total number of rows: 25697

Table truncated, full table size 1030 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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