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Sample GSM666015 Query DataSets for GSM666015
Status Public on May 03, 2012
Title Esophageal squamous cell carcinoma 24
Sample type RNA
 
Channel 1
Source name Esophageal squamous cell carcinoma, fresh frozen tissue
Organism Homo sapiens
Characteristics tissue: Esophageal squamous cell carcinoma tumor
biomaterial: Fresh frozen tissue
gender: Male
tumor stage: IV
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol (Invitrogen) according to the manufacures recommendations. RNA integrity was checked on a Bioanalyzer (Agilent).
Label Cy3
Label protocol Direct labeled by random priming (Promega, Pronto)
 
Channel 2
Source name Human Reference RNA (Stratagene), pool of ten cell lines
Organism Homo sapiens
Characteristics biomaterial: Pool of 10 different cell lines
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol (Invitrogen) according to the manufacures recommendations. RNA integrity was checked on a Bioanalyzer (Agilent).
Label Cy5
Label protocol Direct labeled by random priming (Promega, Pronto)
 
 
Hybridization protocol Hybridizations were performed in 4 x SSC, 0.1% SDS, 0,5 NOTE human 0,8-1,0 µg/µl Cot-1 DNA at 42degC overnight. Arrays were washed three times 4xSSC RT, twice 2xSSC / 0.1% SDS at 42degC, one time 0.2xSSC, and one time 0.1xSSC RT.
Scan protocol Arrays were scanned in Agilent scanner (Agilent Technologies)
Description Esophageal cancer
Data processing Image analysis was performed using Genepix v4.0. Result files were uploaded into BASE where they were filtered and normalized.
Correction of background intensities of Cy3 and Cy5 were calculated by means of a median-feature and median-local background intensities of the uploaded files. The intensity ratios for the individual probes were calculated as intensity of the background-corrected tumor channel (Cy3) divided with the intensity of the background-corrected reference channel (Cy5). Low-quality spots flagged during image analysis, and spots with a signal-to-noise ratio (SNR) of <1.5 and >10% saturation were excluded from further analysis. To compensate for dye bias and local background effects, data were normalized by a lowess algorithm in BASE
 
Submission date Feb 03, 2011
Last update date May 03, 2012
Contact name Anna Isinger Ekstrand
E-mail(s) anna.ekstrand@med.lu.se
Organization name Clinical Sciences
Department Oncology
Street address Barngatan 2b
City Lund
ZIP/Postal code 221 85
Country Sweden
 
Platform ID GPL5886
Series (1)
GSE27040 Dermatan sulfate is involved in the tumorigenic properties of Esophagus Squamous Cell Carcinoma

Data table header descriptions
ID_REF
VALUE normalized log2ratio (ch1/ch2)

Data table
ID_REF VALUE
3738 -0.445913684
11739 -0.517691976
14864 -1.284681308
26779 -1.751569567
26853 -0.992205195
27409 0.808542866
6798 1.097763786
9951 0.248195725
26501 -1.403348866
6112 0.741695876
13551 0.213430201
5387 -0.890908613
17999 0.767603259
26049 0.183473471
20979 -0.43618376
6233
22361 -0.236572215
9976 0.652152243
7755 0.393030813
14366 -1.008963286

Total number of rows: 18785

Table truncated, full table size 310 Kbytes.




Supplementary file Size Download File type/resource
GSM666015_24_GI.txt.gz 2.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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