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Sample GSM667539 Query DataSets for GSM667539
Status Public on Dec 31, 2011
Title Sick Mac 2
Sample type RNA
 
Source name Macrophages from nephritic, lupus nephritis kidneys
Organism Mus musculus
Characteristics cell type: F4/80hi
strain: NZB/W
tissue: kidney
sample time: 2-6 weeks after proteinuria onset, but before the development of terminal renal failure
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using picopure RNA isolation kit (Arcturus Molecular Device Corporation, Sunnyvale, CA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol Refer to the Affymetrix gene expression manual
Scan protocol GeneChip Scanner 3000 7G
Description F4/80hi cell from NZB/W kidney (2-6 weeks after proteinuria onset, but before the development of terminal renal failure)
Data processing The data were analyzed using GenePattern pipeline: RMA method and Mouse Entrez Gene custom CDF annotation version 10 were used for the normalization.
 
Submission date Feb 03, 2011
Last update date Dec 31, 2011
Contact name Celine C Berthier
Organization name University of Michigan
Street address 1150 W. Medical Center Dr, MSRB2
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platform ID GPL7546
Series (1)
GSE27045 Expression data from macrophages isolated from lupus NZB/W mouse kidneys prenephritic, nephritic or after complete remission.

Data table header descriptions
ID_REF
VALUE log2 expression values

Data table
ID_REF VALUE
11287_at 4.873202527
11298_at 5.607696588
11302_at 6.421937773
11303_at 7.006939226
11304_at 7.383481457
11305_at 7.143412651
11307_at 12.52322901
11308_at 11.36657181
11350_at 8.076421885
11352_at 8.65975454
11354_at 4.765659852
11363_at 11.11967151
11364_at 11.55526002
11370_at 11.20958233
11409_at 9.019896968
11416_at 9.798013022
11418_at 4.689947341
11419_at 5.134632013
11421_at 7.828197955
11423_at 4.984335812

Total number of rows: 16539

Table truncated, full table size 343 Kbytes.




Supplementary file Size Download File type/resource
GSM667539.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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