|
| Status |
Public on Mar 01, 2011 |
| Title |
p50KO MEFs LPS 1hr |
| Sample type |
RNA |
| |
|
| Source name |
Primary mouse embryonic fibroblasts (MEFs)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: MEF
genotype/variation: p50KO
agent: LPS
time: 1hr
|
| Treatment protocol |
MEFs were stimulated with LPS or IFNb
|
| Growth protocol |
MEF cells were maintained in DMEM supplemented with 10% bovine calf serum (BCS) to passages 5 to 6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from MEFs with Rneasy columns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion Message Amp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
MEF expression in response to LPS or IFNb
|
| Data processing |
Quantile normalized using Illumina Inc. BeadStudio
|
| |
|
| Submission date |
Feb 07, 2011 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Alexander Hoffmann |
| E-mail(s) |
ahoffmann@ucsd.edu
|
| Phone |
858-822-4670
|
| URL |
http://signalingsystems.ucsd.edu/
|
| Organization name |
UCSD
|
| Department |
Chemistry and Biochemistry
|
| Lab |
UCSD Signaling Systems Laboratory
|
| Street address |
UCSD m/c 0375, NSB 3318 9500, Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0375 |
| Country |
USA |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE27112 |
The specifcity of innate immune response is enforced by repression of interferon-response elements by NFkB p50 |
|
