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Sample GSM669581 Query DataSets for GSM669581
Status Public on Mar 01, 2011
Title WT MEFs IFNb 8hr
Sample type RNA
 
Source name Primary mouse embryonic fibroblasts (MEFs)
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: MEF
genotype/variation: wild type
agent: IFNb
time: 8hr
Treatment protocol MEFs were stimulated with LPS or IFNb
Growth protocol MEF cells were maintained in DMEM supplemented with 10% bovine calf serum (BCS) to passages 5 to 6
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from MEFs with Rneasy columns (Qiagen)
Label biotin
Label protocol Biotinylated cRNA was prepared with the Ambion Message Amp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description MEF expression in response to LPS or IFNb
Data processing Quantile normalized using Illumina Inc. BeadStudio
 
Submission date Feb 07, 2011
Last update date Mar 01, 2011
Contact name Alexander Hoffmann
E-mail(s) ahoffmann@ucsd.edu
Phone 858-822-4670
URL http://signalingsystems.ucsd.edu/
Organization name UCSD
Department Chemistry and Biochemistry
Lab UCSD Signaling Systems Laboratory
Street address UCSD m/c 0375, NSB 3318 9500, Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92093-0375
Country USA
 
Platform ID GPL6103
Series (1)
GSE27112 The specifcity of innate immune response is enforced by repression of interferon-response elements by NFkB p50

Data table header descriptions
ID_REF
VALUE Quantile normalized signal

Data table
ID_REF VALUE
ILMN_1238136 43.3
ILMN_2721178 324.6
ILMN_2773981 1553.2
ILMN_2771349 1417.9
ILMN_2634566 620.8
ILMN_2737647 43.3
ILMN_2744574 45.5
ILMN_2739275 49.4
ILMN_2734484 258.1
ILMN_2699078 40.8
ILMN_1213681 140.5
ILMN_2735415 51.4
ILMN_1237980 37.6
ILMN_3162350 68
ILMN_2712923 98.2
ILMN_2670314 165.4
ILMN_2637698 2782.1
ILMN_2674228 181.3
ILMN_2438555 208.4
ILMN_2601546 92.1

Total number of rows: 24599

Table truncated, full table size 438 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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