|
| Status |
Public on Nov 15, 2012 |
| Title |
undiff (5091212009_B) |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129X1/SvJ x 129S1
age: day 0 differentiation
cell type: embryonic stem cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser by Asuragen Inc.
|
| Label |
biotin
|
| Label protocol |
Total RNA (100ng per sample) was used for preparation of biotin-labeled targets (cRNA) using a MessageAmpTM II-based protocol (Ambion,Inc Austin TX) and one round of amplification
|
| |
|
| Hybridization protocol |
Labeled cRNA was used to probe MouseRef-8 v2 Expression beadchips using Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
biological_replicates: undifferentiated replicate 2
|
| Data processing |
Raw data were extracted using an Illumina BeadArray Reader
Following quality assessment, data from the replicate beads on each array were summarized into raw intensity values with and without background subtraction.
The background subtraction, expression summary, normalization, and log base 2 transformation of gene signals were carried out using Quantile Normalization
|
| |
|
| Submission date |
Feb 07, 2011 |
| Last update date |
Nov 15, 2012 |
| Contact name |
Irina Fernandez |
| E-mail(s) |
irinafer@mail.utexas.edu
|
| Phone |
512-7506072
|
| Organization name |
University of Texas at Austin
|
| Department |
Biomedical Engineering
|
| Lab |
Dr.Krishnendu Roy
|
| Street address |
107 West Dean Keeton st
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE27116 |
cDNA microarray analysis of mouse embryonic stem cells-derived hematopoietic progenitors compared to native hematopoietic stem cells |
|
