|
| Status |
Public on Oct 04, 2005 |
| Title |
Organ culture 20E-treated replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
whole animal
|
| Organism |
Drosophila melanogaster |
| Characteristics |
w1118
|
| Treatment protocol |
Eight animals were dissected in a 9 well glass dish (Corning) and cultured in ~100 µl oxygenated Schneiders Drosophila Medium (Gibco) at 25°C. Cultures were incubated in an a styrofoam box under a constant flow of oxygen. Following an initial incubation of 1 hour, the medium was removed and replaced with fresh Schneiders Drosophila Medium plus 5x10-6 M 20-hydroxyecdysone and incubated for 6 hours at 25°C.
|
| Growth protocol |
Larvae were staged by addition of 0.05% bromophenol blue to food and collected as actively wandering third instar larvae with partial blue guts (~8 hour prior to pupariation).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using TriPure (Roche) followed by further purification with RNAeasy (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Approximately 12 ug of total RNA was processed to produce biotinylated cRNA targest
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
20E treated organ cultures
|
| Data processing |
dChip 1.2
|
| |
|
| Submission date |
Aug 04, 2005 |
| Last update date |
Sep 02, 2005 |
| Contact name |
Robert B Beckstead |
| E-mail(s) |
robertb@uga.edu
|
| Phone |
706-542-0387
|
| Fax |
706-542-1827
|
| Organization name |
The University of Georgia
|
| Department |
Poultry Science
|
| Lab |
Robert B. Beckstead
|
| Street address |
119 Poultry Science Building
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602-2772 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE3060 |
Identification of 20E-regulated genes in Drosophila cultured larval organs |
|
