|
| Status |
Public on Jun 01, 2011 |
| Title |
Ago2-IP RNA Wildtype-1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary chondroyctes; Wt; Ago2-IP
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Primary chondrocytes
genotype: Wt
antibody: Ago2 antibody
genetic background: 129sv/C57 mixed background, backcrossed to C57B6 twice, C57B6 dominant
antibody manufacturer: Wako biochemicals
antibody catalog #: 292-67301
antibody lot #: PEK486
|
| Growth protocol |
Primary rib chondroyctes overnight culture in 10 cm dish in 10%FCD-containing DMEM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by SurePrep (Fisher). Ago2 immunoprecipitation was performed using WAKO's microRNA isolation Kit, mouse Ago2
|
| Label |
biotin
|
| Label protocol |
Ovation Pico + Affymetrix starndard
|
| |
|
| Hybridization protocol |
Affymetrix Standard protocol
|
| Scan protocol |
Affymetrix Standard protocol
|
| Data processing |
Microarray suite 5
|
| |
|
| Submission date |
Feb 09, 2011 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Tatsuya Kobayashi |
| E-mail(s) |
kobayash@helix.mgh.harvard.edu
|
| Phone |
617 7263966
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Endocrine Unit
|
| Lab |
Kobayashi
|
| Street address |
50 Blossom Street, Thier 1101
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE27177 |
RNA profiles of total RNA and Ago2-immunoprecipitated RNA |
|
