|
Status |
Public on Oct 04, 2005 |
Title |
Organ culture cycloheximide-treated replicate 1 |
Sample type |
RNA |
|
|
Source name |
whole animal
|
Organism |
Drosophila melanogaster |
Characteristics |
w1118
|
Treatment protocol |
Eight animals were dissected in a 9 well glass dish (Corning) and cultured in ~100 µl oxygenated Schneiders Drosophila Medium (Gibco) at 25°C. Cultures were incubated in an a styrofoam box under a constant flow of oxygen. Following an initial incubation of 1 hour, the medium was removed and replaced with fresh Schneiders Drosophila Medium plus 8.5x10-5 M cycloheximide and incubated for 6 hours at 25°C.
|
Growth protocol |
Larvae were staged by addition of 0.05% bromophenol blue to food and collected as actively wandering third instar larvae with partial blue guts (~8 hour prior to pupariation).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using TriPure (Roche) followed by further purification with RNAeasy (Qiagen)
|
Label |
biotin
|
Label protocol |
Approximately 12 ug of total RNA was processed to produce biotinylated cRNA targest
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Cycloheximide treated organ cultures
|
Data processing |
dChip 1.2
|
|
|
Submission date |
Aug 04, 2005 |
Last update date |
Sep 02, 2005 |
Contact name |
Robert B Beckstead |
E-mail(s) |
robertb@uga.edu
|
Phone |
706-542-0387
|
Fax |
706-542-1827
|
Organization name |
The University of Georgia
|
Department |
Poultry Science
|
Lab |
Robert B. Beckstead
|
Street address |
119 Poultry Science Building
|
City |
Athens |
State/province |
GA |
ZIP/Postal code |
30602-2772 |
Country |
USA |
|
|
Platform ID |
GPL72 |
Series (1) |
GSE3060 |
Identification of 20E-regulated genes in Drosophila cultured larval organs |
|