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Sample GSM67170 Query DataSets for GSM67170
Status Public on Oct 04, 2005
Title Organ culture cycloheximide-treated replicate 1
Sample type RNA
 
Source name whole animal
Organism Drosophila melanogaster
Characteristics w1118
Treatment protocol Eight animals were dissected in a 9 well glass dish (Corning) and cultured in ~100 µl oxygenated Schneiders Drosophila Medium (Gibco) at 25°C. Cultures were incubated in an a styrofoam box under a constant flow of oxygen. Following an initial incubation of 1 hour, the medium was removed and replaced with fresh Schneiders Drosophila Medium plus 8.5x10-5 M cycloheximide and incubated for 6 hours at 25°C.
Growth protocol Larvae were staged by addition of 0.05% bromophenol blue to food and collected as actively wandering third instar larvae with partial blue guts (~8 hour prior to pupariation).
Extracted molecule total RNA
Extraction protocol RNA was isolated using TriPure (Roche) followed by further purification with RNAeasy (Qiagen)
Label biotin
Label protocol Approximately 12 ug of total RNA was processed to produce biotinylated cRNA targest
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Cycloheximide treated organ cultures
Data processing dChip 1.2
 
Submission date Aug 04, 2005
Last update date Sep 02, 2005
Contact name Robert B Beckstead
E-mail(s) robertb@uga.edu
Phone 706-542-0387
Fax 706-542-1827
Organization name The University of Georgia
Department Poultry Science
Lab Robert B. Beckstead
Street address 119 Poultry Science Building
City Athens
State/province GA
ZIP/Postal code 30602-2772
Country USA
 
Platform ID GPL72
Series (1)
GSE3060 Identification of 20E-regulated genes in Drosophila cultured larval organs

Data table header descriptions
ID_REF
VALUE dChip derived PM-only model gene expression

Data table
ID_REF VALUE
142121_at 240.74
142122_at 161.5
142123_at 150.94
142124_at 108.38
142125_at 32.56
142126_at 81.74
142127_at 74.75
142128_at 87.64
142129_at 9.29
142130_at 91.71
142131_at 44.11
142132_at 76.35
142133_at 202.05
142134_at 110.67
142135_at 77.15
142136_at 158.86
142137_at 77.94
142138_at 172.14
142139_at 135.97
142140_at 197.27

Total number of rows: 13966

Table truncated, full table size 225 Kbytes.




Supplementary file Size Download File type/resource
GSM67170.CEL.CEL.gz 2.7 Mb (ftp)(http) CEL

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