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Sample GSM672499 Query DataSets for GSM672499
Status Public on Sep 08, 2011
Title gavin_OSCA_6_Canine_2
Sample type RNA
 
Source name metastatic osteosarcoma cell line
Organism Canis lupus familiaris
Characteristics cell line: metastatic osteosarcoma
breed: Rhodesian Ridgeback
Sex: M
age at diagnosis: 2.39
treatment: PALL
survival: 0.36
Growth protocol Primary tumor cells or cryopreserved cultured cells. Snap-frozen primary tissue used for 6 samples.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNeasy Mini Kit and QIAshredder column (Qiagen, Valencia, CA).
Label Biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing CEL files were normalized using the GC-RMA algorithm as implemented in the Genedata Analyst Software Package.
 
Submission date Feb 10, 2011
Last update date Sep 08, 2011
Contact name Aaron Sarver
Organization name University of Minnesota
Department Masonic Cancer Center
Lab Biostatistics and Informatics
Street address 425 East River Road
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platform ID GPL3738
Series (1)
GSE27217 Canine osteosarcoma cell lines and tissues

Data table header descriptions
ID_REF
VALUE GC-RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 270.00677
AFFX-BioB-M_at 554.4106
AFFX-BioB-3_at 342.04428
AFFX-BioC-5_at 748.30743
AFFX-BioC-3_at 1232.3699
AFFX-BioDn-5_at 2610.8738
AFFX-BioDn-3_at 2689.5168
AFFX-CreX-5_at 12401.955
AFFX-CreX-3_at 12812.46
AFFX-DapX-5_at 3.3081155
AFFX-DapX-M_at 3.5180776
AFFX-DapX-3_at 3.5957203
AFFX-LysX-5_at 3.713251
AFFX-LysX-M_at 2.7495754
AFFX-LysX-3_at 4.5137267
AFFX-PheX-5_at 4.1418786
AFFX-PheX-M_at 3.0941541
AFFX-PheX-3_at 7.0985904
AFFX-ThrX-5_at 5.886276
AFFX-ThrX-M_at 4.883462

Total number of rows: 43035

Table truncated, full table size 1259 Kbytes.




Supplementary file Size Download File type/resource
GSM672499.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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