|
| Status |
Public on Sep 08, 2011 |
| Title |
gavin_OSCA_11_Canine_2 |
| Sample type |
RNA |
| |
|
| Source name |
metastatic osteosarcoma cell line
|
| Organism |
Canis lupus familiaris |
| Characteristics |
cell line: metastatic osteosarcoma
breed: Rottweiler
Sex: F
age at diagnosis: 5
|
| Growth protocol |
Primary tumor cells or cryopreserved cultured cells. Snap-frozen primary tissue used for 6 samples.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the RNeasy Mini Kit and QIAshredder column (Qiagen, Valencia, CA).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
CEL files were normalized using the GC-RMA algorithm as implemented in the Genedata Analyst Software Package.
|
| |
|
| Submission date |
Feb 10, 2011 |
| Last update date |
Sep 08, 2011 |
| Contact name |
Aaron Sarver |
| Organization name |
University of Minnesota
|
| Department |
Masonic Cancer Center
|
| Lab |
Biostatistics and Informatics
|
| Street address |
425 East River Road
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE27217 |
Canine osteosarcoma cell lines and tissues |
|
