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Sample GSM672519 Query DataSets for GSM672519
Status Public on Sep 08, 2011
Title scott_5_shiloh_Canine_2
Sample type RNA
 
Source name primary osteosarcoma tissue
Organism Canis lupus familiaris
Characteristics tissue: primary osteosarcoma
breed: Belgian tervuren
Sex: MC
age at diagnosis: 6.1
treatment: SOC
survival: 4
Growth protocol Primary tumor cells or cryopreserved cultured cells. Snap-frozen primary tissue used for 6 samples.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNeasy Mini Kit and QIAshredder column (Qiagen, Valencia, CA).
Label Biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing CEL files were normalized using the GC-RMA algorithm as implemented in the Genedata Analyst Software Package.
 
Submission date Feb 10, 2011
Last update date Sep 08, 2011
Contact name Aaron Sarver
Organization name University of Minnesota
Department Masonic Cancer Center
Lab Biostatistics and Informatics
Street address 425 East River Road
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platform ID GPL3738
Series (1)
GSE27217 Canine osteosarcoma cell lines and tissues

Data table header descriptions
ID_REF
VALUE GC-RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 156.36151
AFFX-BioB-M_at 421.21732
AFFX-BioB-3_at 130.98662
AFFX-BioC-5_at 532.73553
AFFX-BioC-3_at 1042.9822
AFFX-BioDn-5_at 3128.1904
AFFX-BioDn-3_at 2707.5566
AFFX-CreX-5_at 16727.309
AFFX-CreX-3_at 16824.842
AFFX-DapX-5_at 2.881908
AFFX-DapX-M_at 2.9706724
AFFX-DapX-3_at 3.6397421
AFFX-LysX-5_at 3.5040298
AFFX-LysX-M_at 2.9398527
AFFX-LysX-3_at 3.6547406
AFFX-PheX-5_at 3.7260358
AFFX-PheX-M_at 2.9311
AFFX-PheX-3_at 6.6493044
AFFX-ThrX-5_at 4.802355
AFFX-ThrX-M_at 4.2381315

Total number of rows: 43035

Table truncated, full table size 1260 Kbytes.




Supplementary file Size Download File type/resource
GSM672519.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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