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Sample GSM6731 Query DataSets for GSM6731
Status Public on May 30, 2003
Title FKS1(5251) #1
Sample type RNA
 
Source name Total RNA
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Description The fks1 mutant strain (5251) was grown overnight in YPD medium, diluted 1:50 and grown to the mid-log phase (OD600 1.6-1.8) and harvested by centrifugation. Total RNA was isolated by a hot phenol procedure (Schmitt et al. 1990, Nucleic Acids Research 18: 3091-3092). Target probe preparation was done according to Affymetrix Gene Expression Technical Manual (Affymetrix, Santa Clara CA). Briefly, first strand and double-stranded cDNA were synthesized from total RNA (10-40 ug) (SuperScript II RT). cDNA was converted to biotinylated complementary RNA by in vitro transcription containing T7 RNA polymerase (Enzo Biochem). The cRNA product was cleaned up on RNeasy spin columns (Qiagen) followed by quantitation spectrophotometrically (UV lambda=260 nm) and by gel electrophoresis. About 20 ug of cRNA was fragmented to yield 35-200 bases fragments before hybridization. RNA probes were hybridized to the entire yeast genome microarray (YG-S98, Affymetrix) for 16 hours at 45 C. After washing in a non-stringent and stringent wash buffer, the probe arrays were stained with streptavidin phycoerythrin in the GeneChip Fluidics Station and scanned with the Affymetrix GeneChip Scanner according to manufacturer’s instructions. Gene chips were analyzed and normalized by GeneChip Analysis Suite.
Keywords = saccharomyces chitin glucosamine
 
Submission date May 23, 2003
Last update date Oct 28, 2005
Contact name Garrett M Frampton
E-mail(s) frampton@mit.edu
Organization name Whitehead Instute - MIT
Department Biology
Lab Richard Young
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL90
Series (1)
GSE441 Yeast glucosamine treatment

Data table header descriptions
ID_REF
VALUE Affymetrix MAS 5.0 signal intensity (scaled to 500 on all genes)
ABS_CALL Affymetrix MAS 5.0 Detection Call (default setting)

Data table
ID_REF VALUE ABS_CALL
10000_at 14.5 P
10001_at 1052.2 P
10002_i_at 2382.3 A
10003_f_at 7722.6 P
10004_at 16.8 A
10005_at 371.5 P
10006_at 127.1 P
10007_at 1103 P
10008_at 4.9 A
10009_at 9.7 A
10010_at 2017.1 P
10011_at 24.2 A
10012_at 341.8 P
10013_at 88.2 P
10014_at 379.6 P
10015_at 5009.6 P
10016_at 1149.2 P
10017_at 3.6 A
10018_at 7.6 A
10019_at 2447.8 P

Total number of rows: 9335

Table truncated, full table size 146 Kbytes.




Supplementary data files not provided

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