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Sample GSM67333 Query DataSets for GSM67333
Status Public on Oct 24, 2005
Title triCH1flox2_1_hypoxia
Sample type RNA
Source name primary mouse embryonic fibroblasts
Organism Mus musculus
Characteristics CBP, p300, CH1, TAZ1, hypoxia, MEF
Extracted molecule total RNA
Label biotin
Description Primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 embryos that contain a deletion in the CH1 domain of both alleles of CBP and one allele of p300. The remaining allele of p300 was a conditional knock out allele. At passage 3 MEFs were infected with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated 0.1% oxygen (hypoxia), with 5% carbon dioxide at 37 C in a humid chamber for 6hrs. At the start of treatment, medium was removed and replaced with medium (DMEM+10% FBS+pen-strep+ l-glu) that had been preequilibrated overnight in hypoxia. Immediately after treatment, cells were lysed in Trizol for RNA extraction.
Sample Type: Mouse Total RNA
Project: Brindle
Sample User: jmorris
Probe Array Type: Mouse430_2
Barcode: @52002900410450110805400482905884
Probe Array Lot: 4004829
Expiration Date: Nov 08 2005 12:00AM
Experiment User: jmorris
Algorithm: Statistical
HZ:4 VZ:4,BG:4,SmoothFactorBG:100,Epsilon:0.5,BF:,Alpha1:0.05,Alpha2:0.065,Tau:0.015,Gamma1H:0.0025,Gamma1L:0.0025,Gamma2H:0.003,Gamma2L:0.003,Perturbation:1.1,TGT:500,NF:1.000000000000,SF:3.852353096008,SFGene:All
Data processing standard Affymetrix protocols
Submission date Aug 05, 2005
Last update date Oct 24, 2005
Contact name Paul K. Brindle
Phone 901 495 2451
Organization name St Jude Children's Research Hospital
Department Biochemistry
Street address 332 N Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platform ID GPL1261
Series (2)
GSE3196 Gene expression in hypoxic MEFs having only p300 and CBP with deleted CH1 domains
GSE3318 Two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression

Data table header descriptions
ID_REF As defined by Affymetrix, these are the probe set identifiers, each of which is unique to a specific probe set defining a specific region of a single gene or set.
VALUE This is the final calculated meauremeent for each probe set identifer that has been made comparable, through scaling or normalization, accross all samples and rows.
DETECTION P-VALUE Defines the confidence in the calculated signal accurately reflecting the expression level of the specific gene or est that the probe set identifies.

Data table
1415670_at 5473.7 0.000244
1415671_at 5348.2 0.000244
1415672_at 4845.9 0.000244
1415673_at 1675.7 0.000732
1415674_a_at 3468.1 0.000244
1415675_at 1307.8 0.000244
1415676_a_at 7450.4 0.000244
1415677_at 716.2 0.000244
1415678_at 2623.6 0.000244
1415679_at 3549.6 0.000244
1415680_at 2427.9 0.000244
1415681_at 2162.9 0.000244
1415682_at 657.4 0.000244
1415683_at 5612.8 0.000244
1415684_at 566.4 0.000244
1415685_at 944.6 0.000244
1415686_at 1649.3 0.000244
1415687_a_at 9070.1 0.000244
1415688_at 5346.3 0.000244
1415689_s_at 333.6 0.000244

Total number of rows: 45101

Table truncated, full table size 1138 Kbytes.

Supplementary data files not provided

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