GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM673388 Query DataSets for GSM673388
Status Public on Dec 01, 2012
Title heart_Scn5a-1798insD/+ mutant [4700266031_D]
Sample type RNA
Source name left ventricle_free-wall
Organism Mus musculus
Characteristics tissue: cardiac
background strain: F2-(129P2/OlaHsdxFVBN/J)
genotype: Scn5a1798insD/+
Treatment protocol None
Growth protocol All mice were supplied with the same SDS diet (SDS CRM(E) PL; Special Diets Services, UK) and water ad libitum and maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the QIAGEN RNeasy mini kit. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
Label biotin
Label protocol Synthesis, amplification and purification of anti-sense RNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion) following the Illumina Sentrix Array Matrix expression protocol.
Hybridization protocol A total of 750 ng biotinylated cRNA was hybridized in accordance with standard Illumina protocols.
Scan protocol Standard Illumina scan protocol
Description F2 hybrid
Data processing Illumina’s default pre-processing steps were performed using beadarray. A variance-stabilization transformation was applied to the summarized data and the resulting data was quantile normalized.
Submission date Feb 11, 2011
Last update date Dec 01, 2012
Contact name Brendon Paul Scicluna
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
Platform ID GPL6885
Series (1)
GSE27236 Gene expression analysis of cardiac left-ventricle tissue from hybrid mice harboring the Scn5a-1798insD/+ mutation

Data table header descriptions
Detection Pval 4700266031_D

Data table
ID_REF VALUE Detection Pval 4700266031_D
ILMN_1250052 8.163696309 0
ILMN_3122480 7.817561448 0.655927835
ILMN_2599935 8.649126144 0
ILMN_2675543 7.819672334 0.630154639
ILMN_2686883 7.82353307 0.587628866
ILMN_2751818 7.867864294 0.260309278
ILMN_2728634 7.986831322 0.005154639
ILMN_3040515 7.806857386 0.777061856
ILMN_2711608 7.872749013 0.233247423
ILMN_1232875 7.781080041 0.932989691
ILMN_1258507 7.804826053 0.792525773
ILMN_2746142 7.833194698 0.5
ILMN_1252690 7.959959942 0.023195876
ILMN_2655499 7.783241155 0.930412371
ILMN_1252870 7.884961196 0.197164948
ILMN_1248179 7.801542318 0.81314433
ILMN_2649955 8.180487935 0
ILMN_2628708 7.791742974 0.889175258
ILMN_3024781 7.811192945 0.728092784
ILMN_2705628 7.814698401 0.694587629

Total number of rows: 25697

Table truncated, full table size 833 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap