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Sample GSM67342 Query DataSets for GSM67342
Status Public on Oct 24, 2005
Title flox1_TSA
Sample type RNA
Source name primary mouse embryonic fibroblasts
Organism Mus musculus
Characteristics CBP, p300, CH1, TAZ1, dipyridyl, hypoxia mimetic, Trichostatin A, TSA, MEF
Extracted molecule total RNA
Label biotin
Description Primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 embryos bearing one conditional knockout allele of CBP and were infected at passage 3 with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated under normoxia with 5% carbon dioxide at 37 C in a humid chamber with 100ng/ml Trichostatin A (TSA), then with EtOH vehicle after 30min. 3 hrs later, cells were lysed in Trizol for RNA extraction.
Sample Type: Mouse Total RNA
Project: Brindle
Sample User: jmorris
Probe Array Type: Mouse430_2
Barcode: @52002900572821040206400907920947
Probe Array Lot: 4009079
Expiration Date: Apr 02 2006 12:00AM
Sample Provider:
Developmental State:
Cell Type:
Genetic Modifications:
Genetic Characteristics:
Disease State:
Reference to Clinical Data:
Growth Conditions:
in vivo Treatments:
Tissue Harvesting Method:
Extract Method:
Molecule Type:
Label Incorporation:
Lab Protocol: Affy Protocol
Experiment User: jmorris
Algorithm: Statistical
HZ:4 VZ:4,BG:2,SmoothFactorBG:100,Epsilon:0.5,BF:,Alpha1:0.05,Alpha2:0.065,Tau:0.015,Gamma1H:0.0025,Gamma1L:0.0025,Gamma2H:0.003,Gamma2L:0.003,Perturbation:1.1,TGT:500,NF:1.000000000000,SF:4.720140933990,SFGene:All
Data processing standard Affymetrix protocols
Submission date Aug 05, 2005
Last update date Oct 24, 2005
Contact name Paul K. Brindle
Phone 901 495 2451
Organization name St Jude Children's Research Hospital
Department Biochemistry
Street address 332 N Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platform ID GPL1261
Series (2)
GSE3296 Gene expression in MEFs in response to treatment with dipyridyl and trichostatin A
GSE3318 Two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression

Data table header descriptions
ID_REF As defined by Affymetrix, these are the probe set identifiers, each of which is unique to a specific probe set defining a specific region of a single gene or set.
VALUE This is the final calculated meauremeent for each probe set identifer that has been made comparable, through scaling or normalization, accross all samples and rows.
DETECTION P-VALUE Defines the confidence in the calculated signal accurately reflecting the expression level of the specific gene or est that the probe set identifies.

Data table
1415670_at 4219.8 0.000244
1415671_at 5451.5 0.000244
1415672_at 3887.5 0.000244
1415673_at 1420.8 0.000732
1415674_a_at 2091.9 0.000244
1415675_at 1152.8 0.000244
1415676_a_at 5911.8 0.000244
1415677_at 1066.7 0.000732
1415678_at 1921.4 0.000244
1415679_at 2476.8 0.000244
1415680_at 1345.8 0.000244
1415681_at 2011.3 0.000244
1415682_at 660.2 0.000244
1415683_at 4212.4 0.000244
1415684_at 622.5 0.004150
1415685_at 942.3 0.000244
1415686_at 1678.4 0.000244
1415687_a_at 8599.6 0.000244
1415688_at 2152.6 0.000244
1415689_s_at 468.3 0.000732

Total number of rows: 45101

Table truncated, full table size 1152 Kbytes.

Supplementary data files not provided

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