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Sample GSM673425 Query DataSets for GSM673425
Status Public on Dec 01, 2012
Title heart_Scn5a-1798insD/+ mutant [4700266042_A]
Sample type RNA
Source name left ventricle_free-wall
Organism Mus musculus
Characteristics tissue: cardiac
background strain: F2-(129P2/OlaHsdxFVBN/J)
genotype: Scn5a1798insD/+
Treatment protocol None
Growth protocol All mice were supplied with the same SDS diet (SDS CRM(E) PL; Special Diets Services, UK) and water ad libitum and maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the QIAGEN RNeasy mini kit. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
Label biotin
Label protocol Synthesis, amplification and purification of anti-sense RNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion) following the Illumina Sentrix Array Matrix expression protocol.
Hybridization protocol A total of 750 ng biotinylated cRNA was hybridized in accordance with standard Illumina protocols.
Scan protocol Standard Illumina scan protocol
Description F2 hybrid
Data processing Illumina’s default pre-processing steps were performed using beadarray. A variance-stabilization transformation was applied to the summarized data and the resulting data was quantile normalized.
Submission date Feb 11, 2011
Last update date Dec 01, 2012
Contact name Brendon Paul Scicluna
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
Platform ID GPL6885
Series (1)
GSE27236 Gene expression analysis of cardiac left-ventricle tissue from hybrid mice harboring the Scn5a-1798insD/+ mutation

Data table header descriptions
Detection Pval 4700266042_A

Data table
ID_REF VALUE Detection Pval 4700266042_A
ILMN_1250052 8.0233006 0
ILMN_3122480 7.756701645 0.996088657
ILMN_2599935 8.837139563 0
ILMN_2675543 7.842035475 0.430247718
ILMN_2686883 7.875024836 0.18904824
ILMN_2751818 7.834906094 0.485006519
ILMN_2728634 7.986962865 0
ILMN_3040515 7.843345488 0.414602347
ILMN_2711608 7.866668954 0.252933507
ILMN_1232875 7.77128732 0.979139505
ILMN_1258507 7.868442903 0.235984355
ILMN_2746142 7.838812679 0.451108214
ILMN_1252690 7.927376265 0.04041721
ILMN_2655499 7.807035132 0.757496741
ILMN_1252870 7.943743371 0.016949153
ILMN_1248179 7.794212116 0.87874837
ILMN_2649955 8.280893956 0
ILMN_2628708 7.837635771 0.460234681
ILMN_3024781 7.854656915 0.329856584
ILMN_2705628 7.8190033 0.641460235

Total number of rows: 25697

Table truncated, full table size 825 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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