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Sample GSM673427 Query DataSets for GSM673427
Status Public on Dec 01, 2012
Title heart_Scn5a-1798insD/+ mutant [4700266042_C]
Sample type RNA
 
Source name left ventricle_free-wall
Organism Mus musculus
Characteristics tissue: cardiac
background strain: F2-(129P2/OlaHsdxFVBN/J)
genotype: Scn5a1798insD/+
Treatment protocol None
Growth protocol All mice were supplied with the same SDS diet (SDS CRM(E) PL; Special Diets Services, UK) and water ad libitum and maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the QIAGEN RNeasy mini kit. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
Label biotin
Label protocol Synthesis, amplification and purification of anti-sense RNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion) following the Illumina Sentrix Array Matrix expression protocol.
 
Hybridization protocol A total of 750 ng biotinylated cRNA was hybridized in accordance with standard Illumina protocols.
Scan protocol Standard Illumina scan protocol
Description F2 hybrid
Data processing Illumina’s default pre-processing steps were performed using beadarray. A variance-stabilization transformation was applied to the summarized data and the resulting data was quantile normalized.
 
Submission date Feb 11, 2011
Last update date Dec 01, 2012
Contact name Brendon Scicluna
E-mail(s) brendon.scicluna@um.edu.mt
Organization name University of Malta
Street address Msida campus
City Msida
ZIP/Postal code MSD2020
Country Malta
 
Platform ID GPL6885
Series (1)
GSE27236 Gene expression analysis of cardiac left-ventricle tissue from hybrid mice harboring the Scn5a-1798insD/+ mutation

Data table header descriptions
ID_REF
VALUE VST-QUANTILE normalized
Detection Pval 4700266042_C

Data table
ID_REF VALUE Detection Pval 4700266042_C
ILMN_1250052 7.997838799 0
ILMN_3122480 7.839671637 0.433639947
ILMN_2599935 8.816478708 0
ILMN_2675543 7.878589642 0.169513798
ILMN_2686883 7.818847559 0.633377135
ILMN_2751818 7.785648613 0.925098555
ILMN_2728634 7.890568117 0.134034166
ILMN_3040515 7.854629599 0.304862024
ILMN_2711608 7.876712542 0.174770039
ILMN_1232875 7.811197359 0.714848883
ILMN_1258507 7.809221387 0.737187911
ILMN_2746142 7.839634513 0.433639947
ILMN_1252690 7.854434665 0.306176084
ILMN_2655499 7.832835186 0.486202365
ILMN_1252870 7.942285906 0.022339028
ILMN_1248179 7.787349454 0.913272011
ILMN_2649955 8.22243247 0
ILMN_2628708 7.795746652 0.867279895
ILMN_3024781 7.807230452 0.754270696
ILMN_2705628 7.818255518 0.643889619

Total number of rows: 25697

Table truncated, full table size 825 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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