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Sample GSM673445 Query DataSets for GSM673445
Status Public on Dec 01, 2012
Title heart_Scn5a-1798insD/+ mutant [4700266048_E]
Sample type RNA
 
Source name left ventricle_free-wall
Organism Mus musculus
Characteristics tissue: cardiac
background strain: F2-(129P2/OlaHsdxFVBN/J)
genotype: Scn5a1798insD/+
Treatment protocol None
Growth protocol All mice were supplied with the same SDS diet (SDS CRM(E) PL; Special Diets Services, UK) and water ad libitum and maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the QIAGEN RNeasy mini kit. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
Label biotin
Label protocol Synthesis, amplification and purification of anti-sense RNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion) following the Illumina Sentrix Array Matrix expression protocol.
 
Hybridization protocol A total of 750 ng biotinylated cRNA was hybridized in accordance with standard Illumina protocols.
Scan protocol Standard Illumina scan protocol
Description F2 hybrid
Data processing Illumina’s default pre-processing steps were performed using beadarray. A variance-stabilization transformation was applied to the summarized data and the resulting data was quantile normalized.
 
Submission date Feb 11, 2011
Last update date Dec 01, 2012
Contact name Brendon Paul Scicluna
E-mail(s) b.scicluna@amsterdamumc.nl
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE27236 Gene expression analysis of cardiac left-ventricle tissue from hybrid mice harboring the Scn5a-1798insD/+ mutation

Data table header descriptions
ID_REF
VALUE VST-QUANTILE normalized
Detection Pval 4700266048_E

Data table
ID_REF VALUE Detection Pval 4700266048_E
ILMN_1250052 8.072763152 0
ILMN_3122480 7.829303599 0.532536521
ILMN_2599935 8.564168118 0
ILMN_2675543 7.827084136 0.555112882
ILMN_2686883 7.806291119 0.791500664
ILMN_2751818 7.830333317 0.521912351
ILMN_2728634 7.91465031 0.058432935
ILMN_3040515 7.821735252 0.622841965
ILMN_2711608 7.851190212 0.335989376
ILMN_1232875 7.817309854 0.69189907
ILMN_1258507 7.82219846 0.620185923
ILMN_2746142 7.84180502 0.414342629
ILMN_1252690 7.872352648 0.220451527
ILMN_2655499 7.836603032 0.462151394
ILMN_1252870 8.010564961 0
ILMN_1248179 7.846062097 0.378486056
ILMN_2649955 8.204445813 0
ILMN_2628708 7.880213041 0.183266932
ILMN_3024781 7.863124712 0.257636122
ILMN_2705628 7.817414615 0.690571049

Total number of rows: 25697

Table truncated, full table size 825 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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