|
| Status |
Public on Dec 01, 2012 |
| Title |
heart_Scn5a-1798insD/+ mutant [4700266053_E] |
| Sample type |
RNA |
| |
|
| Source name |
left ventricle_free-wall
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cardiac
background strain: F2-(129P2/OlaHsdxFVBN/J)
genotype: Scn5a1798insD/+
|
| Treatment protocol |
None
|
| Growth protocol |
All mice were supplied with the same SDS diet (SDS CRM(E) PL; Special Diets Services, UK) and water ad libitum and maintained on a 12-hour light/dark cycle in a temperature and humidity controlled environment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy mini kit. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity (RIN>9.0) of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent).
|
| Label |
biotin
|
| Label protocol |
Synthesis, amplification and purification of anti-sense RNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion) following the Illumina Sentrix Array Matrix expression protocol.
|
| |
|
| Hybridization protocol |
A total of 750 ng biotinylated cRNA was hybridized in accordance with standard Illumina protocols.
|
| Scan protocol |
Standard Illumina scan protocol
|
| Description |
F2 hybrid
|
| Data processing |
Illumina’s default pre-processing steps were performed using beadarray. A variance-stabilization transformation was applied to the summarized data and the resulting data was quantile normalized.
|
| |
|
| Submission date |
Feb 11, 2011 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE27236 |
Gene expression analysis of cardiac left-ventricle tissue from hybrid mice harboring the Scn5a-1798insD/+ mutation |
|
