NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM67347 Query DataSets for GSM67347
Status Public on Oct 24, 2005
Title triCH1flox1_DP
Sample type RNA
 
Source name primary mouse embryonic fibroblasts
Organism Mus musculus
Characteristics CBP, p300, CH1, TAZ1, dipyridyl, hypoxia mimetic, Trichostatin A, TSA, MEF
Extracted molecule total RNA
Label biotin
 
Description Primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 embryos that contain a deletion in the CH1 domain of both alleles of p300 and one allele of CBP. The remaining allele of CBP was a conditional knock out allele. At passage 3 MEFs were infected with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated under normoxia with 5% carbon dioxide at 37 C in a humid chamber with EtOH vehicle, then with 100um dipyridyl (DP) after 30min. 3 hrs later, cells were lysed in Trizol for RNA extraction.
Sample Type: Mouse Total RNA
Project: Brindle
Sample User: jmorris
Probe Array Type: Mouse430_2
Barcode: @52002900572820040206400907920905
Probe Array Lot: 4009079
Expiration Date: Apr 02 2006 12:00AM
Genus:
species:
Strain/Line:
Individual:
Sample Provider:
Sex:
Age:
Developmental State:
Tissue:
Cell Type:
Genetic Modifications:
Genetic Characteristics:
Disease State:
Reference to Clinical Data:
Growth Conditions:
in vivo Treatments:
Tissue Harvesting Method:
Extract Method:
Molecule Type:
Amplification:
Amount:
Label:
Label Incorporation:
Lab Protocol: Affy Protocol
Experiment User: jmorris
Algorithm: Statistical
HZ:4 VZ:4,BG:2,SmoothFactorBG:100,Epsilon:0.5,BF:,Alpha1:0.05,Alpha2:0.065,Tau:0.015,Gamma1H:0.0025,Gamma1L:0.0025,Gamma2H:0.003,Gamma2L:0.003,Perturbation:1.1,TGT:500,NF:1.000000000000,SF:5.238101482391,SFGene:All
Background:Avg:129.93,Stdev:3.11,Max:139.4,Min:118.6
Noise:Avg:9.03,Stdev:0.29,Max:9.7,Min:8.4
RawQ:4.76
Corner+:Avg:525,Count:32
Corner-:Avg:9326,Count:32
Central-:Avg:10178,Count:9
Data processing standard Affymetrix protocols
 
Submission date Aug 05, 2005
Last update date Oct 24, 2005
Contact name Paul K. Brindle
E-mail(s) paul.brindle@stjude.org
Phone 901 495 2451
Organization name St Jude Children's Research Hospital
Department Biochemistry
Street address 332 N Lauderdale
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL1261
Series (2)
GSE3296 Gene expression in MEFs in response to treatment with dipyridyl and trichostatin A
GSE3318 Two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression

Data table header descriptions
ID_REF As defined by Affymetrix, these are the probe set identifiers, each of which is unique to a specific probe set defining a specific region of a single gene or set.
VALUE This is the final calculated meauremeent for each probe set identifer that has been made comparable, through scaling or normalization, accross all samples and rows.
DETECTION P-VALUE Defines the confidence in the calculated signal accurately reflecting the expression level of the specific gene or est that the probe set identifies.

Data table
ID_REF VALUE DETECTION P-VALUE
1415670_at 3493.6 0.000244
1415671_at 3884.3 0.000732
1415672_at 4306.1 0.000244
1415673_at 1568.7 0.000732
1415674_a_at 2070.8 0.000244
1415675_at 1053.8 0.000244
1415676_a_at 5957.7 0.000244
1415677_at 601.9 0.001221
1415678_at 2963.9 0.000244
1415679_at 2523.9 0.000244
1415680_at 2018.0 0.000244
1415681_at 1732.0 0.000244
1415682_at 661.8 0.000244
1415683_at 5221.1 0.000244
1415684_at 487.3 0.004150
1415685_at 1018.2 0.000244
1415686_at 2462.0 0.000244
1415687_a_at 7659.0 0.000244
1415688_at 4019.5 0.000244
1415689_s_at 490.9 0.001953

Total number of rows: 45101

Table truncated, full table size 1148 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap