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Sample GSM67362 Query DataSets for GSM67362
Status Public on Sep 26, 2005
Title Control replicate 1
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control, untreated
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control, untreated
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 296.4 P 0.000857
AFFX-BioB-M_at 466.5 P 0.000195
AFFX-BioB-3_at 187.8 P 0.000258
AFFX-BioC-5_at 664.7 P 0.000081
AFFX-BioC-3_at 544.1 P 0.000052
AFFX-BioDn-5_at 746.1 P 0.000052
AFFX-BioDn-3_at 3421.5 P 0.00011
AFFX-CreX-5_at 7898.3 P 0.000052
AFFX-CreX-3_at 9437.8 P 0.000044
AFFX-DapX-5_at 14107.5 P 0.000044
AFFX-DapX-M_at 13765.9 P 0.000044
AFFX-DapX-3_at 15172.5 P 0.000044
AFFX-LysX-5_at 1.6 A 0.876428
AFFX-LysX-M_at 21.1 A 0.51489
AFFX-LysX-3_at 21.8 A 0.165861
AFFX-PheX-5_at 1.8 A 0.932339
AFFX-PheX-M_at 1.2 A 0.986189
AFFX-PheX-3_at 26.7 A 0.470241
AFFX-ThrX-5_at 3.9 A 0.910522
AFFX-ThrX-M_at 2.8 A 0.749204

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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