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Sample GSM67363 Query DataSets for GSM67363
Status Public on Sep 26, 2005
Title Control replicate 2
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control, untreated
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control, untreated
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 179.7 P 0.000754
AFFX-BioB-M_at 313.4 P 0.000446
AFFX-BioB-3_at 166.5 P 0.000581
AFFX-BioC-5_at 493.2 P 0.000169
AFFX-BioC-3_at 443.5 P 0.000052
AFFX-BioDn-5_at 535.2 P 0.000044
AFFX-BioDn-3_at 2983.1 P 0.000147
AFFX-CreX-5_at 5893.9 P 0.000052
AFFX-CreX-3_at 7547.4 P 0.000044
AFFX-DapX-5_at 11499.8 P 0.000044
AFFX-DapX-M_at 11993.6 P 0.000044
AFFX-DapX-3_at 12789.8 P 0.000044
AFFX-LysX-5_at 11.3 A 0.470241
AFFX-LysX-M_at 23.1 A 0.672921
AFFX-LysX-3_at 11.3 A 0.250796
AFFX-PheX-5_at 1.4 A 0.921998
AFFX-PheX-M_at 1.9 A 0.978098
AFFX-PheX-3_at 9.2 A 0.645547
AFFX-ThrX-5_at 5.2 A 0.932322
AFFX-ThrX-M_at 4.7 A 0.760956

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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