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Sample GSM67365 Query DataSets for GSM67365
Status Public on Sep 26, 2005
Title Control replicate 4
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control, untreated
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control, untreated
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 306.9 P 0.000754
AFFX-BioB-M_at 485.7 P 0.000195
AFFX-BioB-3_at 241 P 0.000147
AFFX-BioC-5_at 710.6 P 0.000147
AFFX-BioC-3_at 577.7 P 0.000052
AFFX-BioDn-5_at 723.4 P 0.000044
AFFX-BioDn-3_at 3529.5 P 0.000081
AFFX-CreX-5_at 7937.6 P 0.000052
AFFX-CreX-3_at 10061.3 P 0.000044
AFFX-DapX-5_at 14451.2 P 0.000044
AFFX-DapX-M_at 15039 P 0.000044
AFFX-DapX-3_at 15965 P 0.000044
AFFX-LysX-5_at 6.2 A 0.340661
AFFX-LysX-M_at 6 A 0.712257
AFFX-LysX-3_at 18.7 A 0.131361
AFFX-PheX-5_at 2 A 0.916408
AFFX-PheX-M_at 2 A 0.973889
AFFX-PheX-3_at 18.1 A 0.52976
AFFX-ThrX-5_at 3.4 A 0.921998
AFFX-ThrX-M_at 4 A 0.737173

Total number of rows: 45101

Table truncated, full table size 1205 Kbytes.

Supplementary data files not provided

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