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Status
Public on Sep 26, 2005
Title
Control replicate 5
Sample type
RNA
Source name
Mouse heart
Organism
Mus musculus Characteristics
Control, untreated
Extracted molecule
total RNA
Extraction protocol
Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Control, untreated
Data processing
Robust Multichip Analysis (RMA)
Submission date
Aug 05, 2005
Last update date
Sep 26, 2005
Contact name
Norman Buroker
E-mail(s)
Norman.Buroker@seattlechildrens.org Organization name
Seattle Children's Hospital
Lab
Portman
Street address
4800 Sand Point Way N.E.
City
Seattle
State/province
WA
ZIP/Postal code
98105
Country
USA
Platform ID
GPL1261 Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
183.2
P
0.000754
AFFX-BioB-M_at
317.1
P
0.000258
AFFX-BioB-3_at
108.7
P
0.000857
AFFX-BioC-5_at
456.3
P
0.000169
AFFX-BioC-3_at
409.1
P
0.000044
AFFX-BioDn-5_at
600.7
P
0.00006
AFFX-BioDn-3_at
2593.2
P
0.000258
AFFX-CreX-5_at
6372
P
0.000052
AFFX-CreX-3_at
7381.4
P
0.000044
AFFX-DapX-5_at
11553.9
P
0.000044
AFFX-DapX-M_at
12040.7
P
0.000044
AFFX-DapX-3_at
12869.4
P
0.000044
AFFX-LysX-5_at
2.3
A
0.659339
AFFX-LysX-M_at
23.2
A
0.5
AFFX-LysX-3_at
22.5
M
0.058444
AFFX-PheX-5_at
1.9
A
0.949771
AFFX-PheX-M_at
2.7
A
0.921998
AFFX-PheX-3_at
6.9
A
0.617401
AFFX-ThrX-5_at
2.9
A
0.916408
AFFX-ThrX-M_at
6.9
A
0.645547
45101 1204 Kbytes .Supplementary data files not provided
