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Sample GSM67366 Query DataSets for GSM67366
Status Public on Sep 26, 2005
Title Control replicate 5
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control, untreated
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control, untreated
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 183.2 P 0.000754
AFFX-BioB-M_at 317.1 P 0.000258
AFFX-BioB-3_at 108.7 P 0.000857
AFFX-BioC-5_at 456.3 P 0.000169
AFFX-BioC-3_at 409.1 P 0.000044
AFFX-BioDn-5_at 600.7 P 0.00006
AFFX-BioDn-3_at 2593.2 P 0.000258
AFFX-CreX-5_at 6372 P 0.000052
AFFX-CreX-3_at 7381.4 P 0.000044
AFFX-DapX-5_at 11553.9 P 0.000044
AFFX-DapX-M_at 12040.7 P 0.000044
AFFX-DapX-3_at 12869.4 P 0.000044
AFFX-LysX-5_at 2.3 A 0.659339
AFFX-LysX-M_at 23.2 A 0.5
AFFX-LysX-3_at 22.5 M 0.058444
AFFX-PheX-5_at 1.9 A 0.949771
AFFX-PheX-M_at 2.7 A 0.921998
AFFX-PheX-3_at 6.9 A 0.617401
AFFX-ThrX-5_at 2.9 A 0.916408
AFFX-ThrX-M_at 6.9 A 0.645547

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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