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Sample GSM67367 Query DataSets for GSM67367
Status Public on Sep 26, 2005
Title Control replicate 6
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control, untreated
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control, untreated
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 218.2 P 0.000754
AFFX-BioB-M_at 426 P 0.000297
AFFX-BioB-3_at 186.4 P 0.00034
AFFX-BioC-5_at 579.9 P 0.000127
AFFX-BioC-3_at 448.2 P 0.000052
AFFX-BioDn-5_at 705.1 P 0.000052
AFFX-BioDn-3_at 3054.1 P 0.000169
AFFX-CreX-5_at 7497.4 P 0.000052
AFFX-CreX-3_at 7937 P 0.000044
AFFX-DapX-5_at 10449.4 P 0.000044
AFFX-DapX-M_at 12077.6 P 0.000044
AFFX-DapX-3_at 12645.3 P 0.000044
AFFX-LysX-5_at 3 A 0.588627
AFFX-LysX-M_at 34.5 A 0.58862
AFFX-LysX-3_at 7.2 A 0.175328
AFFX-PheX-5_at 1.6 A 0.937071
AFFX-PheX-M_at 2.1 A 0.966323
AFFX-PheX-3_at 22.4 A 0.544587
AFFX-ThrX-5_at 8.2 A 0.783496
AFFX-ThrX-M_at 3.6 A 0.617401

Total number of rows: 45101

Table truncated, full table size 1205 Kbytes.

Supplementary data files not provided

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