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Sample GSM67368 Query DataSets for GSM67368
Status Public on Sep 26, 2005
Title Control replicate 7
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control, untreated
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control, untreated
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 277.8 P 0.000856
AFFX-BioB-M_at 445.8 P 0.000225
AFFX-BioB-3_at 207.3 P 0.000445
AFFX-BioC-5_at 650 P 0.000095
AFFX-BioC-3_at 575.7 P 0.000044
AFFX-BioDn-5_at 729 P 0.000044
AFFX-BioDn-3_at 3627.9 P 0.000081
AFFX-CreX-5_at 8750.8 P 0.000044
AFFX-CreX-3_at 9934.7 P 0.000044
AFFX-DapX-5_at 10664.8 P 0.000044
AFFX-DapX-M_at 14001.9 P 0.000044
AFFX-DapX-3_at 15681.1 P 0.000044
AFFX-LysX-5_at 18.2 A 0.574038
AFFX-LysX-M_at 7.4 A 0.916408
AFFX-LysX-3_at 22 A 0.39692
AFFX-PheX-5_at 2.7 A 0.814869
AFFX-PheX-M_at 2 A 0.993146
AFFX-PheX-3_at 45.8 A 0.262827
AFFX-ThrX-5_at 7.3 A 0.937071
AFFX-ThrX-M_at 1.7 A 0.910522

Total number of rows: 45101

Table truncated, full table size 1205 Kbytes.

Supplementary data files not provided

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