|
| Status |
Public on Feb 11, 2011 |
| Title |
Ferret whole blood, unvaccinated, on Day 1, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Ferret
|
| Organism |
Mustela putorius furo |
| Characteristics |
tissue: Whole Blood
treatment group: unvaccinated
|
| Treatment protocol |
Ferrets were immunized with each adjuvanted vaccine through intramuscular rout by 200ul total volume in one leg. At day 0 and day 1 post immunization, whole blood was collected in Paxgene tube for later RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA from lung was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from ferret whole blood, unvaccinated
|
| Data processing |
The data were analyzed with PLIER using Expression Console Version 1.1 (Affymetrix) default analysis settings.
|
| |
|
| Submission date |
Feb 11, 2011 |
| Last update date |
Feb 11, 2011 |
| Contact name |
Longsi Ran |
| E-mail(s) |
lran@uhnresearch.ca
|
| Phone |
416-581-7607
|
| Organization name |
Toronto General Research Institute
|
| Department |
Division of Experimental Therapeutics
|
| Lab |
Dr. David J Kelvin
|
| Street address |
101 College Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE27248 |
Molecular Characterization of In Vivo Adjuvant Activity in Ferrets Vaccinated against Influenza Virus |
|
