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Sample GSM67369 Query DataSets for GSM67369
Status Public on Sep 26, 2005
Title del337TR replicate 1
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics deletion in 337TR
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description deletion in 337TR
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 245.3 P 0.001102
AFFX-BioB-M_at 251.3 P 0.000225
AFFX-BioB-3_at 158.5 P 0.00141
AFFX-BioC-5_at 488.3 P 0.00011
AFFX-BioC-3_at 379.3 P 0.000052
AFFX-BioDn-5_at 584 P 0.00006
AFFX-BioDn-3_at 2959.2 P 0.000258
AFFX-CreX-5_at 6714.2 P 0.000052
AFFX-CreX-3_at 7825.4 P 0.000044
AFFX-DapX-5_at 27.5 A 0.131361
AFFX-DapX-M_at 29.4 A 0.175328
AFFX-DapX-3_at 55.3 A 0.089478
AFFX-LysX-5_at 3.3 A 0.470241
AFFX-LysX-M_at 21.8 A 0.58862
AFFX-LysX-3_at 27.4 P 0.030976
AFFX-PheX-5_at 829.9 P 0.000044
AFFX-PheX-M_at 1058.8 P 0.00006
AFFX-PheX-3_at 1755.5 P 0.000044
AFFX-ThrX-5_at 25.5 A 0.672921
AFFX-ThrX-M_at 6.6 A 0.631562

Total number of rows: 45101

Table truncated, full table size 1206 Kbytes.

Supplementary data files not provided

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