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Sample GSM67371 Query DataSets for GSM67371
Status Public on Sep 26, 2005
Title del337TR replicate 3
Sample type RNA
 
Source name Mouse heart
Organism Mus musculus
Characteristics deletion in 337TR
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description deletion in 337TR
Data processing Robust Multichip Analysis (RMA)
 
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
E-mail(s) Norman.Buroker@seattlechildrens.org
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
 
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 284.3 P 0.000754
AFFX-BioB-M_at 460.9 P 0.000195
AFFX-BioB-3_at 225.5 P 0.000225
AFFX-BioC-5_at 673.8 P 0.000147
AFFX-BioC-3_at 612.6 P 0.000052
AFFX-BioDn-5_at 785.9 P 0.000044
AFFX-BioDn-3_at 3749.3 P 0.000081
AFFX-CreX-5_at 9354 P 0.000052
AFFX-CreX-3_at 10572.8 P 0.000044
AFFX-DapX-5_at 15.6 A 0.239063
AFFX-DapX-M_at 31.8 A 0.131361
AFFX-DapX-3_at 35 A 0.131361
AFFX-LysX-5_at 1.6 A 0.52976
AFFX-LysX-M_at 8.9 A 0.544587
AFFX-LysX-3_at 23.4 A 0.165861
AFFX-PheX-5_at 5455.6 P 0.000044
AFFX-PheX-M_at 5410.9 P 0.000044
AFFX-PheX-3_at 7791.6 P 0.000044
AFFX-ThrX-5_at 6.3 A 0.868639
AFFX-ThrX-M_at 5.7 A 0.659339

Total number of rows: 45101

Table truncated, full table size 1203 Kbytes.




Supplementary data files not provided

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