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Sample GSM67372 Query DataSets for GSM67372
Status Public on Sep 26, 2005
Title del337TR replicate 4
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics deletion in 337TR
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description deletion in 337TR
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 261.1 P 0.000857
AFFX-BioB-M_at 367.4 P 0.000258
AFFX-BioB-3_at 195.3 P 0.000662
AFFX-BioC-5_at 583.3 P 0.000127
AFFX-BioC-3_at 447.5 P 0.00006
AFFX-BioDn-5_at 675.5 P 0.000052
AFFX-BioDn-3_at 3252.9 P 0.000127
AFFX-CreX-5_at 7838.8 P 0.000052
AFFX-CreX-3_at 8814.5 P 0.000044
AFFX-DapX-5_at 9.9 A 0.440646
AFFX-DapX-M_at 32.9 A 0.185131
AFFX-DapX-3_at 54.4 M 0.058444
AFFX-LysX-5_at 2.6 A 0.631562
AFFX-LysX-M_at 53.8 A 0.368438
AFFX-LysX-3_at 63.6 P 0.00039
AFFX-PheX-5_at 3685.9 P 0.000044
AFFX-PheX-M_at 3635.4 P 0.000044
AFFX-PheX-3_at 5799.5 P 0.000044
AFFX-ThrX-5_at 6.1 A 0.672921
AFFX-ThrX-M_at 2.5 A 0.953533

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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