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Sample GSM67373 Query DataSets for GSM67373
Status Public on Sep 26, 2005
Title del337TR replicate 5
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics deletion in 337TR
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description deletion in 337TR
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 269.2 P 0.000754
AFFX-BioB-M_at 464.6 P 0.000147
AFFX-BioB-3_at 230.9 P 0.000754
AFFX-BioC-5_at 739.2 P 0.000081
AFFX-BioC-3_at 565.6 P 0.000044
AFFX-BioDn-5_at 751 P 0.000044
AFFX-BioDn-3_at 4110 P 0.000127
AFFX-CreX-5_at 10543.1 P 0.000052
AFFX-CreX-3_at 11188.1 P 0.000044
AFFX-DapX-5_at 30.4 A 0.108959
AFFX-DapX-M_at 20 A 0.39692
AFFX-DapX-3_at 43.4 A 0.205732
AFFX-LysX-5_at 3.4 A 0.686292
AFFX-LysX-M_at 10.5 A 0.686277
AFFX-LysX-3_at 37.5 A 0.095667
AFFX-PheX-5_at 1793.8 P 0.000044
AFFX-PheX-M_at 2159.1 P 0.000052
AFFX-PheX-3_at 3606.1 P 0.000044
AFFX-ThrX-5_at 10.2 A 0.749223
AFFX-ThrX-M_at 3.7 A 0.760937

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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