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Sample GSM67374 Query DataSets for GSM67374
Status Public on Sep 26, 2005
Title del337TR replicate 6
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics deletion in 337TR
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description deletion in 337TR
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 286.4 P 0.000754
AFFX-BioB-M_at 377.6 P 0.000258
AFFX-BioB-3_at 184.8 P 0.000081
AFFX-BioC-5_at 566.6 P 0.00011
AFFX-BioC-3_at 539.3 P 0.000044
AFFX-BioDn-5_at 694.7 P 0.000044
AFFX-BioDn-3_at 3216.4 P 0.000147
AFFX-CreX-5_at 8514.2 P 0.000052
AFFX-CreX-3_at 9180.6 P 0.000044
AFFX-DapX-5_at 14.5 A 0.156732
AFFX-DapX-M_at 27.2 A 0.108979
AFFX-DapX-3_at 28.8 A 0.131361
AFFX-LysX-5_at 2.4 A 0.659339
AFFX-LysX-M_at 29.4 A 0.440646
AFFX-LysX-3_at 9.7 A 0.41138
AFFX-PheX-5_at 2491.6 P 0.000044
AFFX-PheX-M_at 2862.8 P 0.000044
AFFX-PheX-3_at 4667.1 P 0.000044
AFFX-ThrX-5_at 2 A 0.957038
AFFX-ThrX-M_at 7.2 A 0.659339

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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