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Sample GSM67374

Query DataSets for GSM67374

Status

Public on Sep 26, 2005

Title

del337TR replicate 6

Sample type

RNA

Source name

Mouse heart

Organism

Mus musculus

Characteristics

deletion in 337TR

Extracted molecule

total RNA

Extraction protocol

Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

deletion in 337TR

Data processing

Robust Multichip Analysis (RMA)

Submission date

Aug 05, 2005

Last update date

Sep 26, 2005

Contact name

Norman Buroker

E-mail(s)

Norman.Buroker@seattlechildrens.org

Organization name

Seattle Children's Hospital

Lab

Portman

Street address

4800 Sand Point Way N.E.

City

Seattle

State/province

ZIP/Postal code

98105

Country

USA

Platform ID

GPL1261

Series (1)

GSE3067

Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	286.4	P	0.000754
AFFX-BioB-M_at	377.6	P	0.000258
AFFX-BioB-3_at	184.8	P	0.000081
AFFX-BioC-5_at	566.6	P	0.00011
AFFX-BioC-3_at	539.3	P	0.000044
AFFX-BioDn-5_at	694.7	P	0.000044
AFFX-BioDn-3_at	3216.4	P	0.000147
AFFX-CreX-5_at	8514.2	P	0.000052
AFFX-CreX-3_at	9180.6	P	0.000044
AFFX-DapX-5_at	14.5	A	0.156732
AFFX-DapX-M_at	27.2	A	0.108979
AFFX-DapX-3_at	28.8	A	0.131361
AFFX-LysX-5_at	2.4	A	0.659339
AFFX-LysX-M_at	29.4	A	0.440646
AFFX-LysX-3_at	9.7	A	0.41138
AFFX-PheX-5_at	2491.6	P	0.000044
AFFX-PheX-M_at	2862.8	P	0.000044
AFFX-PheX-3_at	4667.1	P	0.000044
AFFX-ThrX-5_at	2	A	0.957038
AFFX-ThrX-M_at	7.2	A	0.659339

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided